Beeler LabBehavioral and physiological investigations of dopamine
We mostly use mouse genetic models, including knockouts, knockdowns and tissue specific deletions. More recently, we have begun using newly available mouse genetic tools, such as early immediate gene (IEG) systems to drive selective expression of cre—and cre-dependent gene expression– in active neurons during a behavioral epoch. We also use pharmacological models as appropriate.
We use a range of behavioral methods from simple paradigms, such as open field and accelerating rotarod, to more sophisticated testing, including homecage operant paradigms. We utilize genetic and pharmacological manipulations and use computational approaches to model more complex datasets generated by homecage paradigms.
We use patch-clamp electrophysiology to study synaptic transmission and plasticity in the striatum, particularly the regulation of corticostriatal synaptic plasticity and properties of cholinergic interneurons. To assess dopamine release, we use fast-scan cyclic voltammetry (FSCV). We have two FSCV options: we can test evoked dopamine release in anesthetized animal or we can monitor dopamine release in awake, behaving mice using a chronically implanted carbon fiber electrode. To manipulate specific circuits and cell populations, we use opto- and pharmaco-genetics.